Abstract
Traditional medicine of Brazilian central savannah preconizes Dipteryx alata extracts against snakebites venoms. Previous studies have shown the extract of this plant’s bark antagonizing the neuromuscular blockade caused by snake venom in isolated mice diaphragm and phrenic nerve models. In the present study, the triterpenoids lupeol, lupenone, 28-OH-lupenone and betulin, which are constituents of the plant, were observed by using in vitro pharmacological tests. These tests showed the efficacy of all tested triterpenoids (1 mg/5mL) against the irreversible neuromuscular blockade caused by snake venom from Bothrops jararacussu (Bjssu, 40 μg/mL, n=19), as following: betulin (68 ± 7%, n=11) ~ lupeol (70 ± 8%, n=5) > lupenone (45 ± 9%, n=7) ~ 28-OH-lupenone (54 ± 5%, n=4). Betulin (39.5 ± 9%, n=9) and lupenone (49.5 ± 8%, n=4) significantly protected against Crotalus durissus terrificus (CDT, 10 μg/mL, n=10) envenomation of neuromuscular junction. The mixture of betulin or lupeol + Bjssu venom and betulin or lupenone + CDT venom showed significant phytochemical protection in morphological analysis. Considering both pharmacological and morphological results, betulin showed the best protective effect against neuromuscular blockade and myotoxicity caused by both snake venoms. In addition, it has a potent short-term excitatory action in the neuromuscular junction. We concluded that the studied triterpenoids, especially betulin, showed antiophidian properties.
Current Organic Chemistry
Title:The Effect of Lupane Triterpenoids (Dipteryx alata Vogel) in the in vitro Neuromuscular Blockade and Myotoxicity of two Snake Venoms
Volume: 16 Issue: 22
Author(s): Miriele Cristina Ferraz, Luciane Aparecida Celestino Parrilha, Maria Silvia Duarte Moraes, Jorge Amaral Filho, Jose Carlos Cogo, Marcio Galdino dos Santos, Luiz Madaleno Franco, Francisco Carlos Groppo, Pilar Puebla, Arturo San Feliciano and Yoko Oshima-Franco
Affiliation:
Abstract: Traditional medicine of Brazilian central savannah preconizes Dipteryx alata extracts against snakebites venoms. Previous studies have shown the extract of this plant’s bark antagonizing the neuromuscular blockade caused by snake venom in isolated mice diaphragm and phrenic nerve models. In the present study, the triterpenoids lupeol, lupenone, 28-OH-lupenone and betulin, which are constituents of the plant, were observed by using in vitro pharmacological tests. These tests showed the efficacy of all tested triterpenoids (1 mg/5mL) against the irreversible neuromuscular blockade caused by snake venom from Bothrops jararacussu (Bjssu, 40 μg/mL, n=19), as following: betulin (68 ± 7%, n=11) ~ lupeol (70 ± 8%, n=5) > lupenone (45 ± 9%, n=7) ~ 28-OH-lupenone (54 ± 5%, n=4). Betulin (39.5 ± 9%, n=9) and lupenone (49.5 ± 8%, n=4) significantly protected against Crotalus durissus terrificus (CDT, 10 μg/mL, n=10) envenomation of neuromuscular junction. The mixture of betulin or lupeol + Bjssu venom and betulin or lupenone + CDT venom showed significant phytochemical protection in morphological analysis. Considering both pharmacological and morphological results, betulin showed the best protective effect against neuromuscular blockade and myotoxicity caused by both snake venoms. In addition, it has a potent short-term excitatory action in the neuromuscular junction. We concluded that the studied triterpenoids, especially betulin, showed antiophidian properties.
Export Options
About this article
Cite this article as:
Cristina Ferraz Miriele, Aparecida Celestino Parrilha Luciane, Silvia Duarte Moraes Maria, Amaral Filho Jorge, Carlos Cogo Jose, Galdino dos Santos Marcio, Madaleno Franco Luiz, Carlos Groppo Francisco, Puebla Pilar, San Feliciano Arturo and Oshima-Franco Yoko, The Effect of Lupane Triterpenoids (Dipteryx alata Vogel) in the in vitro Neuromuscular Blockade and Myotoxicity of two Snake Venoms, Current Organic Chemistry 2012; 16 (22) . https://dx.doi.org/10.2174/138527212804004481
DOI https://dx.doi.org/10.2174/138527212804004481 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Neonatal Brain Hemorrhage (NBH) of Prematurity: Translational Mechanisms of the Vascular-Neural Network
Current Medicinal Chemistry Sex Steroid Hormones, Cardiovascular Diseases and The Metabolic Syndrome
Cardiovascular & Hematological Agents in Medicinal Chemistry Fenofibrate: Metabolic and Pleiotropic Effects
Current Vascular Pharmacology Genetic Variations of the Hemostatic System as Risk Factors for Venous and Arterial Thrombotic Disease
Current Genomics An Evaluation of the Efficacy of Local Hemostatic Measures in Dental Patients Taking Oral Anticoagulants: A Critical Review of the Literature Over the Past Two Decades
Current Clinical Pharmacology Patent Selections
Recent Patents on Biomedical Engineering (Discontinued) Novel Anti-Platelets in Stable Coronary Artery Disease
Current Pharmaceutical Design Hyaluronic Acid/Parecoxib-Loaded PLGA Microspheres for Therapy of Temporomandibular Disorders
Current Drug Delivery Withdrawal Notice: Synergistic Effect of Myricetin and Phenolic Acid Derivatives on the Reversal of Dengue Fever Related Thrombocytopenia and its Pharmacokinetics study in Plasma by using HPLC and UPLC-Q-TOF
Current Drug Metabolism Store-Dependent Ca2+ Entry in Endothelial Progenitor Cells As a Perspective Tool to Enhance Cell-Based Therapy and Adverse Tumour Vascularization
Current Medicinal Chemistry Challenges and Promises of Developing Thrombin Receptor Antagonists
Recent Patents on Cardiovascular Drug Discovery Prophylaxis of Venous Thromboembolism in Major Orthopedic Surgery: A Practical Approach
Cardiovascular & Hematological Agents in Medicinal Chemistry Proinflammatory Cytokines and Chemokines in Neonatal Brain Damage
Current Pediatric Reviews PEGylation: a successful approach to improve the biopharmaceutical potential of snake venom thrombin-like serine protease
Protein & Peptide Letters The Impact of Coagulation and Fibrinolysis Cascades on the Development of Septic and Autoimmune Arthritis
Current Rheumatology Reviews Novel Oral Anticoagulants: Recommendations for Patient Evaluation, Treatment Initiation, Follow-up and Perioperative Management
Cardiovascular & Hematological Disorders-Drug Targets The Role of Oxidative Stress in the Pathogenesis of Type 2 Diabetes Mellitus Micro- and Macrovascular Complications: Avenues for a Mechanistic-Based Therapeutic Approach
Current Diabetes Reviews Endothelial Dysfunction: Methods of Assessment and Application to Hypertension
Current Pharmaceutical Design Current Strategies of Tissue Engineering in Talus Chondral Defects
Current Stem Cell Research & Therapy Preface
Current Protein & Peptide Science