Cytokines are known to play a key role in regulation of gastric functions. Interferon-alpha (IFN-α) has been published to impair
gastric motility. Aims of this study were to clarify effect of IFN-α on gastric acid secretion (GAS) and determine role of nitric oxide
(NO) in the process. Both subcutaneous (1000, 10000, 100 000 IU, s.c.) and intracisternal (10, 100, 1000 IU, i.c.) injections of IFN-α
dose-dependently inhibited GAS induced by pylorus ligation in male SD rats in 2 hrs (370±40, 233±39, 208±50 micromol vs control
415±59 micromol and 481±50, 249±75, 141±25 micromol vs control 485±65 micromol, respectively). Central doses inducing same level
inhibition were 100 times lower. NOS inhibitor L-NAME (3 mg/kg, i.v.) blocked the inhibitory effect of i.c. ED50 dose 100 IU IFN-α
(507±75 micromol/2 hrs), while L-arginine, the substrate of nitric oxide synthase (NOS) prevented L-NAME action (266±82 micromol/2
hrs). D-arginine failed to prevent L-NAME action on IFN-α-induced inhibition of GAS. Aminoguanidine, a selective inhibitor of inducible
NOS (iNOS) failed to block IFN-α induced inhibition of GAS.
Results suggest that IFN-α inhibits GAS centrally through nitric oxide pathways probably mediated by continuous isoform of NOS that
can be important in regulation of GAS in healthy or pathological conditions.
Keywords: Brain-gut interactions, cytokines, NOS inhibitors, iNOS, cNOS, peripheral and central interferon-alpha action, vago-vagal reflex, central nervous system, pylorus ligation, D-arginine
Rights & PermissionsPrintExport