Current Nutrition & Food Science

Undurti N. Das  
UND Life Sciences
Shaker Heights
OH 44120
USA

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Structural Characterization of Water-Soluble Polysaccharides from the Fruiting Bodies of Lentinus edodes Mushrooms

Author(s): Irene Palacios, Eva Guillamon, Ana Garcia-Lafuente, Ana Villares.

Abstract:

Water-soluble polysaccharides have been extracted from the fruiting bodies of Lentinus edodes mushrooms. Three carbohydrate fractions (cold water, hot water and hot aqueous NaOH) from the mushrooms were separated by ethanol precipitation. Size exclusion chromatography revealed the presence of a unique polysaccharide in each fraction. Cold water fraction polysaccharide (PLeC) was composed of glucose and galactose bound by (1→3) and (1→4) linkages with a high degree of branching at O-3 and O-4 of galactose residues. Hot water fraction polysaccharide (PLeH) showed (1→4),(1→6)-linked glucopyranosyl residues with branches at O-4 and O-6. Differently, hot aqueous NaOH fraction (PLeB) consisted of a (1→3),(1→6)-linked glucan branched at O-3 and O-6. NMR studies revealed that the polysaccharides PLeC and PLeH displayed both α and β configurations whereas PLeB presented β anomeric configuration. The analysis of molecular arrangement by complexation with Congo red showed that the three polysaccharides (PLeC, PLeH and PLeB) displayed a triple helix conformation.

Keywords: Bioactive carbohydrate, fruiting body, fungi, galactan, glucan, Lentinus edodes, mushroom, polysaccharide, residues, Chromatography

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Article Details

VOLUME: 8
ISSUE: 4
Year: 2012
Page: [235 - 241]
Pages: 7
DOI: 10.2174/157340112803832165
Price: $58