Abstract
Physalia physalis is a marine cnidarian from which high molecular weight toxins with hemolytic and neurotoxic effects have been isolated. In the present work, two novel toxins, PpV9.4 and PpV19.3 were purified from P. physalis by bioactive guideline isolation. It involved two steps of column chromatography, gel filtration and RP-HPLC. The molecular weights were 550.7 and 4720.9 Da for PpV9.4 and PpV19.3, respectively. In the light of the Edman sequencing results, the structure of these toxins included the presence of modified amino acids. Both toxins increased the percentage of insulin secreting beta-cells and induced cytosolic Ca2+ elevation. To date, this is the first report of low molecular weight toxins increasing insulin secretion purified from cnidarians, by constituting a new approach to the study of beta-cells physiology.
Keywords: Animal experiment, cnidarian venom, cytosolic calcium, glucose tolerance test, insulin release, ion channel, islet beta-cell, mass spectrometry, Physalia physalis, toxins
Current Medicinal Chemistry
Title:Toxins from Physalia physalis (Cnidaria) Raise the Intracellular Ca2+ of Beta-Cells and Promote Insulin Secretion
Volume: 19 Issue: 31
Author(s): C.M. Diaz-Garcia, D. Fuentes-Silva, C. Sanchez-Soto, D. Dominguez-Perez, N. Garcia-Delgado, C. Varela, G. Mendoza-Hernandez, A. Rodriguez-Romero, O. Castaneda and M. Hiriart
Affiliation:
Keywords: Animal experiment, cnidarian venom, cytosolic calcium, glucose tolerance test, insulin release, ion channel, islet beta-cell, mass spectrometry, Physalia physalis, toxins
Abstract: Physalia physalis is a marine cnidarian from which high molecular weight toxins with hemolytic and neurotoxic effects have been isolated. In the present work, two novel toxins, PpV9.4 and PpV19.3 were purified from P. physalis by bioactive guideline isolation. It involved two steps of column chromatography, gel filtration and RP-HPLC. The molecular weights were 550.7 and 4720.9 Da for PpV9.4 and PpV19.3, respectively. In the light of the Edman sequencing results, the structure of these toxins included the presence of modified amino acids. Both toxins increased the percentage of insulin secreting beta-cells and induced cytosolic Ca2+ elevation. To date, this is the first report of low molecular weight toxins increasing insulin secretion purified from cnidarians, by constituting a new approach to the study of beta-cells physiology.
Export Options
About this article
Cite this article as:
Diaz-Garcia C.M., Fuentes-Silva D., Sanchez-Soto C., Dominguez-Perez D., Garcia-Delgado N., Varela C., Mendoza-Hernandez G., Rodriguez-Romero A., Castaneda O. and Hiriart M., Toxins from Physalia physalis (Cnidaria) Raise the Intracellular Ca2+ of Beta-Cells and Promote Insulin Secretion, Current Medicinal Chemistry 2012; 19 (31) . https://dx.doi.org/10.2174/092986712803833308
DOI https://dx.doi.org/10.2174/092986712803833308 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Diuretics in the Treatment of Elderly Hypertensive Patients
Cardiovascular & Hematological Agents in Medicinal Chemistry The Role of Prostaglandins in Liver Ischemia-Reperfusion Injury
Current Pharmaceutical Design N-Hydroxyurea and Acyl Nitroso Compounds as Nitroxyl (HNO) and Nitric Oxide (NO) Donors
Current Topics in Medicinal Chemistry Immunochemical Analysis of Endogenous and Exogenous Estrogens
Current Pharmaceutical Analysis Persistent Pulmonary Hypertension of the Newborn: Therapeutical Approach
Mini-Reviews in Medicinal Chemistry Pericyte: Potential Target for Hemorrhagic Stroke Prevention and Treatment
Current Drug Delivery Chronopharmaceutics Based Modern Colon Specific Drug Delivery Systems
Current Drug Discovery Technologies Possible Role of NUCB2/nesfatin-1 in Adipogenesis
Current Pharmaceutical Design Update on Statins: Hope for Osteoporotic Fracture Healing Treatment
Current Drug Targets The Possible Effects of Dapagliflozin on 12-derived Electrocardiogram in Patients with Type 2 Diabetes Mellitus
Endocrine, Metabolic & Immune Disorders - Drug Targets Telmisartan in the Management of Diabetic Nephropathy: A Contemporary View
Current Diabetes Reviews Antisense DNA and RNA: Potential Therapeutics for Viral Infection
Anti-Infective Agents in Medicinal Chemistry MicroRNAs: Modulators of Tooth Development
MicroRNA Nonconvulsive (Dialeptic) Status Epilepticus in Children
Current Pediatric Reviews Recent Advances in the Membrane Receptor Initiated Vitamin D Signaling of Calcium and Phosphate Transport Across Intestinal and Kidney Epithelia
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Prevalence and Risk Factors of Vitamin D Deficiency in Critically Ill Patients
Inflammation & Allergy - Drug Targets (Discontinued) Chondromodulin-I and Tenomodulin: The Negative Control of Angiogenesis in Connective Tissue
Current Pharmaceutical Design Regulation of Inflammatory Responses by Oxidized Phospholipids: Structure-Function Relationships
Current Pharmaceutical Design Emerging Therapy for Diabetic Neuropathy: Cell Therapy Targeting Vessels and Nerves
Endocrine, Metabolic & Immune Disorders - Drug Targets Transcriptional Regulation in Mammalian Pituitary Development and Disease
Current Genomics