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Current Medicinal Chemistry
ISSN (Print): 0929-8673
ISSN (Online): 1875-533X
VOLUME: 19
ISSUE: 31
DOI: 10.2174/092986712803833308      Price:  $58









Toxins from Physalia physalis (Cnidaria) Raise the Intracellular Ca2+ of Beta-Cells and Promote Insulin Secretion

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Author(s): C.M. Diaz-Garcia, D. Fuentes-Silva, C. Sanchez-Soto, D. Dominguez-Perez, N. Garcia-Delgado, C. Varela, G. Mendoza-Hernandez, A. Rodriguez-Romero, O. Castaneda and M. Hiriart
Pages 5414-5423 (10)
Abstract:
Physalia physalis is a marine cnidarian from which high molecular weight toxins with hemolytic and neurotoxic effects have been isolated. In the present work, two novel toxins, PpV9.4 and PpV19.3 were purified from P. physalis by bioactive guideline isolation. It involved two steps of column chromatography, gel filtration and RP-HPLC. The molecular weights were 550.7 and 4720.9 Da for PpV9.4 and PpV19.3, respectively. In the light of the Edman sequencing results, the structure of these toxins included the presence of modified amino acids. Both toxins increased the percentage of insulin secreting beta-cells and induced cytosolic Ca2+ elevation. To date, this is the first report of low molecular weight toxins increasing insulin secretion purified from cnidarians, by constituting a new approach to the study of beta-cells physiology.
Keywords:
Animal experiment, cnidarian venom, cytosolic calcium, glucose tolerance test, insulin release, ion channel, islet beta-cell, mass spectrometry, Physalia physalis, toxins
Affiliation:
Department of Neural Development and Physiology, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico/Circuito Ext. SN, UNAM, Coyoacan, Mexico DF. CP 04510, Mexico.