Making the Most of Pathological Specimens: Molecular Diagnosis in Formalin-Fixed, Paraffin Embedded Tissue

Author(s): Jennifer A. Fairley, Katelyn Gilmour, Kathy Walsh.

Journal Name: Current Drug Targets

Volume 13 , Issue 12 , 2012

Abstract:

The development of commercial reagents designed specifically for use with formalin-fixed paraffin-embedded (FFPE) tissue has unlocked the diagnostic potential of this prolific resource. The availability of archival FFPE tissue and tissue from current patients make it an ideal resource for molecular testing. Despite its stability and ability to preserve morphological information, FFPE provides a number of technical challenges to the study of biomolecules. In particular, the cross-linking and processing present problems in the extraction and isolation of DNA, RNA and protein and affect their use in downstream analysis. Here we will discuss some of the problems of FFPE tissue, how they can be overcome and how FFPE material can be used within clinical molecular diagnostics.

Keywords: Biomarkers, BRAF, EGFR, FFPE tissue, FFPE diagnostics, HER2, KRAS, molecular diagnostics, molecular pathology, personalised medicine, targeted therapy.

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Article Details

VOLUME: 13
ISSUE: 12
Year: 2012
Page: [1475 - 1487]
Pages: 13
DOI: 10.2174/138945012803530125
Price: $58

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