High Resolution Episcopic Microscopy – Current Applications

Stefan      H. Geyer; Timothy      J. Mohun; Lars      P. Kamolz; Wolfgang      J. Weninger

Abstract

High-resolution episcopic microscopy (HREM) is a recently developed technique, which permits the rapid generation of digital volume data from biological material. Typical HREM data have a resolution of approximately 2x2x2 µm3 and are comprised of a stack of inherently aligned images, which resemble the quality of digital images of histological sections. This study aims at recapitulating technical details of the HREM method, providing information on recent modifications and briefly discussing the current and future fields of application.

Keywords: 3D modelling, episcopic microscopy, imaging, visualization, light microscopy, Green fluorescence protein, Yellow Fluorescence Protein, Microtome, embryos, keratinoblasts, mouse embryos, organ morphology, LacZ, up-down positioning, own-movements.