Bioactivity of Exogenous Cytoskeleton Proteins: Regulation of Development of Plant Microspores as Biosensors
Victoria V. Roshchina,
Ivan M. Vikhlyantsev.
Bioactivity of the cytoskeleton proteins such as actin, myosin and titin that have been extracted from the rabbit
muscle was analyzed on plant misrospores as unicellular biosensors. The fibrillar forms of the proteins stimulated the
germination of vegetative microspores from horsetail Equisetum arvense and generative (pollen) microspores from
knight’s star Hippeastrum hybridum. In special experiments the proteins tested were able to reverse the blockade of ion
channels by drugs d-tubocurarine and α-bungarotoxin, acting on cholinoreceptors, or verapamil, regulating Ca2+-channels.
Keywords: Actin, biosensors, Equisetum arvense, germination, Hippeastrum hybridum, ion channels’ blockers, myosin, pollen,
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