Controversies About the Chromosomal Stability of Cultivated Mesenchymal Stem Cells: Their Clinical Use is it Safe?
Reginaldo Justino Ferreira,
Ana Carolina Irioda,
Ricardo Correa Cunha,
Julio Cesar Francisco,
Luiz Cesar Guarita-Souza,
Garikipati Venkata Naga Srikanth,
Juan Carlos Chachques,
Katherine Athayde Teixeira de Carvalho.
The usefulness of adult stem cells in research and therapeutic applications highly relies on their genomic integrity
and stability. Many laboratories including ours have addressed this concern using methods such as karyotyping, Qbanding,
fluorescent in situ hybridization, array CGH, flow cytometry and Pap test to evaluate number and structure of
chromosomes and cellular phenotype. This review attempts to summarize the findings reported so far for the studies on
chromosomal aberrations in adult stem cells and warrant to perform certain basic tests before transplantation to avoid any
adverse reactions, which will thus aid in better therapeutic output after cellular transplantation in the treatment of various
Keywords: Adult stem cell, chromosomal abnormalities, FISH, genetic screening, in vitro transformation, karyotyping, MSC
cultivation, neoplastic transformation of MSC, stem cell (SC) biology.
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