Role of Translationally Regulated Genes in Embryonic Stem Cell Differentiation: Integration of Transcriptome and Translational State Profiling
Pp. 180-192 (13)
Qian Yi Lee, Winston Koh, Prabha Sampath and Vivek Tanavde
This chapter describes a method for genome-wide identification of translationally
regulated genes during embryonic stem cell differentiation using integrated transcriptome and
translation state profiling. Previous attempts at identification of translationally regulated genes
have focused on measuring the fractionation of the mRNA molecules in the translated and
untranslated fractions without considering the transcriptional status of these genes. In this
method we describe how integrating information about transcriptional and translational status
of genes enables in identification of translationally regulated genes with much greater
accuracy. This approach developed for microarrays can also be used for gene expression
measured by next generation sequencing.
Translationally regulated genes, human embryonic stem cell,
diffrentiation, integration, transcriptome, translational state profiling, all-trans
retinoic acid (ATRA).
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