Genomics of Alternative Splicing in Stem Cells
Pp. 147-160 (14)
Stephanie C. Huelga and Gene W. Yeo
Alternative splicing has the ability to expand proteome diversity among
different cell and tissue types, and various stages of differentiation and development. In
higher eukaryotes, alternative pre-mRNA splicing is highly regulated by many cellular
factors and only in the past decade has the magnitude of this co/post-transcriptional
regulation been revealed. Here we review recent technologies that have enabled
genome-wide detection of alternative splicing, and highlight computational methods
that have been developed to identify and understand the interplay between the cis and
trans factors important for regulating alternative splicing in stem cells.
Alternative splicing, spliceosome, exons, introns, pre-mRNA, splicing
factors, splicing regulatory elements (SRE), RNA binding proteins (RBPs), CLIPseq
or HITS-CLIP, RNA-seq, microarrays, transcriptome, genomics, highthroughput
sequencing, splicing code.
Cellular and Molecular Medicine, University of California San Diego, 2880 Torrey Pines Scenic Drive, Rm 3805, La Jolla, CA 92037, USA