Transcriptional Co-Expression Analysis of Embryonic Stem Cells
Pp. 109-132 (24)
Yu Sun and Ming Zhan
The decision between differentiation and self-renewal of embryonic stem
cells (ESCs) are controlled by a complex network formed by interacting genes or
proteins. Identifying critical components of this regulatory network and exploring their
behavior patterns is crucial towards understanding the underlying mechanisms
controlling ESC differentiation and realizing their potentials in regenerative medicine.
In this chapter, we describe the usage of co-expression analysis in identifying conserved
and divergent genomic, transcriptomic, and network modules critical for the earliest
stage of ESCs differentiation.
Co-expression, cell cycle, transcriptome mapping, chromosomal
domains, embryonic stem cell, embryonic bodies, comparative transcriptomics,
transcriptional module, LIF, FGF, Nodal, Wnt, BMP, TGF-β, Oct4, Sox2, nanog,
Department of Systems Medicine and Bioengineering, The Methodist Hospital Research Institute, Houston, TX 77030, USA