Combined Immunization of Mice with DNA, rMVA and rAd5 Expressing HIV-1 Structural Genes from Different Subtypes

Author(s): Lingfei Zhang, Liu Yang, Xia Feng, Zhulun Zhuang, Shuangqing Yu, Ling Yang, Hongxia Li, Xianghui Yu, Wei Kong, Yi Zeng.

Journal Name:Current HIV Research

Volume 10 , Issue 6 , 2012

Abstract:

The objective of present paper is to study the immunogenicity of combinations of multiple vector vaccines expressing HIV-1 structural genes from different subtypes. Mice were vaccinated with DNA (B’/C) and rMVA (B’/C) vaccines expressing B’/C recombinant subtype gag-pol and env genes, DNA (B’) and rAd5 (B’) vaccines expressing subtype B’ gag gene with different combination schemes. HIV-1 Gag-specific cellular immune responses and P24- specific IgG levels were analyzed by IFN-γ enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) respectively. ELISPOT results indicated that the Gag-specific cellular immune responses induced by combination of three vaccines were much higher than that induced by combination of two vaccines. Among the groups of mice immunized with two vaccines, the groups with rAd5 booster elicited higher cellular immune responses compared with the groups with rMVA booster. All the test groups of three vaccines in combination could induce similar level of cellular immune responses, which did not correlate with the immunization order. ELISA results showed that p24- specific IgG induced by combination of three vaccines were much higher than that induced by combination of two vaccines. It indicates that the combination scheme of multiple vector vaccines maybe a promising AIDS vaccine strategy.

Keywords: HIV-1, vaccines, combined immunization, adenovirus, MVA, DNA, AIDS, ELISPOT, ELISA, genes.

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Article Details

VOLUME: 10
ISSUE: 6
Year: 2012
Page: [498 - 503]
Pages: 6
DOI: 10.2174/157016212802429776
Price: $58