Login

Journal Image
Drug Delivery Letters
ISSN (Print): 2210-3031
ISSN (Online): 2210-304X
VOLUME: 2
ISSUE: 2
DOI: 10.2174/2210304x11202020126      Price:  $58









Study of Neonatal Exposure to Estrogenic and Androgenic Endocrine Disruptors by Normal-Phase HPLC

img
Author(s): Masahiro Kuwada, Rei Kawashima, Kazuo Nakamura, Hideyo Hasumi and Jun Maki
Pages 126-131 (6)
Abstract:
Neonatal exposure to estrogenic and androgenic endocrine disruptors induces developmental abnormalities in the female reproductive system. To investigate whether neonatal exposure affects oogenesis in juvenile and pubertal ovary, Sprague-Dawley rat pups were given estrogen or various endocrine disruptors by a single injection on the day of birth at concentrations ranging between 2 mM to 40 mM, and sacrificed on day 21 (juvenile) or 50 (puberty). The ovaries were weighed and examined histologically at each stage. Further, 17 β-estradiol synthesis of oogenesis was analyzed using normal-phase high-performance liquid chromatography. Neonatal exposure to 17 β-estradiol, diethylstilbestrol and estrogen receptor inhibitors significantly reduced ovary weights, although estrogen receptor inhibitors were completely restored by 17 β-estradiol synthesis during puberty.
Keywords:
Endocrine disruptor, neonatal exposure, ovary, oogenesis, 17 β-estradiol, estrogen receptor.
Affiliation:
Department of Nutrition, Faculty of Human Health, Hachinohe University, 13-98 Mihono, Hachinohe, Aomori 031-0844, Japan.