Drug Delivery Letters

Marc Schneider  
Biopharmaceutics and Pharmaceutical Technology
Department of Pharmacy
Saarland University
661213 Saarbrücken


Study of Neonatal Exposure to Estrogenic and Androgenic Endocrine Disruptors by Normal-Phase HPLC

Author(s): Masahiro Kuwada, Rei Kawashima, Kazuo Nakamura, Hideyo Hasumi and Jun Maki

Affiliation: Department of Nutrition, Faculty of Human Health, Hachinohe University, 13-98 Mihono, Hachinohe, Aomori 031-0844, Japan.


Neonatal exposure to estrogenic and androgenic endocrine disruptors induces developmental abnormalities in the female reproductive system. To investigate whether neonatal exposure affects oogenesis in juvenile and pubertal ovary, Sprague-Dawley rat pups were given estrogen or various endocrine disruptors by a single injection on the day of birth at concentrations ranging between 2 mM to 40 mM, and sacrificed on day 21 (juvenile) or 50 (puberty). The ovaries were weighed and examined histologically at each stage. Further, 17 β-estradiol synthesis of oogenesis was analyzed using normal-phase high-performance liquid chromatography. Neonatal exposure to 17 β-estradiol, diethylstilbestrol and estrogen receptor inhibitors significantly reduced ovary weights, although estrogen receptor inhibitors were completely restored by 17 β-estradiol synthesis during puberty.

Keywords: Endocrine disruptor, neonatal exposure, ovary, oogenesis, 17 β-estradiol, estrogen receptor.

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Article Details

Page: [126 - 131]
Pages: 6
DOI: 10.2174/2210304x11202020126