Current Pharmaceutical Analysis

Anastasios Economou
Department of Chemistry
Laboratory of Analytical Chemistry
University of Athens


Quality Control of Fingerprint of Radix Astragali by High-Performance Liquid Chromatography Coupled with Chemometric Methods

Author(s): Zu-fei Feng, Xiao-ming Sun, Sheng-rong Ning and Duo-long Di

Affiliation: Key laboratory of Chemistry of Northwestern Plant Resources & Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No.18, Tianshui Middle Road, Lanzhou, 730000, P.R. China.


High-performance liquid chromatography (HPLC) was employed in a fingerprint analysis of Radix astragali. A characteristic HPLC profile of known Radix astragali samples collected from five primary habitats in China was established by evaluating 15 samples with chemometric methods, including similarity evaluation, hierarchical cluster analysis (HCA), principal component analysis (PCA) and discriminant analysis (DA). The feasibility and advantages of employing chromatographic fingerprinting combined with chemometric methods were investigated and demonstrated in the evaluation of Radix astragali for the first time. The results showed that the chromatographic fingerprint combined with chemometric methods could efficiently distinguish Radix astragali from various areas and provide quality control in the quantitative analysis.

Keywords: Chemometric, Fingerprint, HPLC, Radix astragali, principal component analysis, discriminant analysis, nephritis, diabetes, hypertension, cirrhosis

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Article Details

Page: [225 - 235]
Pages: 11
DOI: 10.2174/157341212801619289