Ganoderma lucidum Polysaccharides Exert Anti-Hyperglycemic Effect on Streptozotocin-Induced Diabetic Rats Through Affecting β-Cells

Author(s): Jusheng Zheng, Bin Yang, Yinghua Yu, Qi Chen, Tao Huang, Duo Li.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 15 , Issue 7 , 2012

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Abstract:

Previous studies have demonstrated that Ganoderma lucidum polysaccharides (Gl-PS) exhibited potential antihyperglycemic effect in rats. The aim of the present study was to investigate the mechanism of the hypoglycemic effect of a low- molecular-weight Gl-PS in streptozotocin (STZ)-induced diabetic Sprague-Dawley (SD) rats. Gl-PS was extracted and purified from Ganodema lucidum fruiting body. 50 male SD rats were included in the study; 10 were taken as healthy controls; 40 were induced to diabetes by a single injection of 65 mg/kg STZ, of which 30 were selected as successful diabetic rat models. The 30 diabetic rats were divided into three groups: Gl-PS (200 mg/kg Gl-PS), metformin (100 mg/kg metformin) and diabetic control (n = 10 per group). After eight weeks’ oral administration, plasma concentrations of fasting glucose, triacylglyceride, total cholesterol and nitric oxide were significantly decreased in Gl-PS and metformin groups. Pancreatic superoxide dismutase, catalase and glutathione peroxidase were significantly increased in Gl-PS and metformin groups. Histopathological results showed that Gl-PS and metformin had protective effect on β-cells. The mRNA expressions of Bcl-2 and PDX-1 in pancreas were up-regulated, but Bax, iNOS and Casp-3 down-regulated in Gl- PS and metformin groups compared to diabetic control group. The present results suggested that Gl-PS had a hypoglycemic effect in STZ-induced diabetic rats through preventing apoptosis of pancreatic β-cells and enhancing β- cells regeneration.

Keywords: Anti-hyperglycemic effect, apoptosis, Bax, Bcl-2, Casp-3, catalase, diabetes, Ganoderma lucidum, glutathione peroxidase, iNOS, mRNA expression, metformin, pancreatic β-cells, PDX-1, polysaccharides, streptozotocin, superoxide dismutase

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Article Details

VOLUME: 15
ISSUE: 7
Year: 2012
Page: [542 - 550]
Pages: 9
DOI: 10.2174/138620712801619168
Price: $58

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